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complete grna screening system  (TaKaRa)


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    TaKaRa complete grna screening system
    Complete Grna Screening System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+grna+screening+system/Guide-it+Complete+sgRNA+Screening+System/10__1007_slash_s11240___025___03198___w-94-28-32
    Average 95 stars, based on 65 article reviews
    complete grna screening system - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Efficient protoplast isolation and transfection for CRISPR/Cas9-based genome editing in pea (Pisum sativum L.)
    Article Snippet: .. For assessing the activity of the selected gRNAs, in-vitro cleavage assay of the PsPDS gene template was performed with transcribed gRNAs and Cas9 nuclease by using the Guide-it Complete gRNA Screening System (Takara Bio, Japan) The DNA cleavage efficiency of gRNAs was observed on 1.5% agarose gel electrophoresis. .. CRISPR/Cas9 plant expression vector for targeting phytoene desaturase (PsPDS) in pea The binary vector pKSE401 (Addgene, Plasmid #62,202), which contains Arabidopsis thaliana U6 promoter (AtU6) for gRNA expression and Zea mays codon-optimized Cas9 (ZmCas9) for efficient editing in pea, was used for designing a multiplex gRNAs construct targeting the first exon 1 3 12 Page 4 of 13 Plant Cell, Tissue and Organ Culture (PCTOC) (2025) 163:12 parameters were subsequently utilized for CRISPR/Cas9based editing of the phytoene desaturase (PsPDS) gene in pea protoplasts.

    Cleavage Assay:

    Article Title: Efficient protoplast isolation and transfection for CRISPR/Cas9-based genome editing in pea (Pisum sativum L.)
    Article Snippet: .. For assessing the activity of the selected gRNAs, in-vitro cleavage assay of the PsPDS gene template was performed with transcribed gRNAs and Cas9 nuclease by using the Guide-it Complete gRNA Screening System (Takara Bio, Japan) The DNA cleavage efficiency of gRNAs was observed on 1.5% agarose gel electrophoresis. .. CRISPR/Cas9 plant expression vector for targeting phytoene desaturase (PsPDS) in pea The binary vector pKSE401 (Addgene, Plasmid #62,202), which contains Arabidopsis thaliana U6 promoter (AtU6) for gRNA expression and Zea mays codon-optimized Cas9 (ZmCas9) for efficient editing in pea, was used for designing a multiplex gRNAs construct targeting the first exon 1 3 12 Page 4 of 13 Plant Cell, Tissue and Organ Culture (PCTOC) (2025) 163:12 parameters were subsequently utilized for CRISPR/Cas9based editing of the phytoene desaturase (PsPDS) gene in pea protoplasts.

    Agarose Gel Electrophoresis:

    Article Title: Efficient protoplast isolation and transfection for CRISPR/Cas9-based genome editing in pea (Pisum sativum L.)
    Article Snippet: .. For assessing the activity of the selected gRNAs, in-vitro cleavage assay of the PsPDS gene template was performed with transcribed gRNAs and Cas9 nuclease by using the Guide-it Complete gRNA Screening System (Takara Bio, Japan) The DNA cleavage efficiency of gRNAs was observed on 1.5% agarose gel electrophoresis. .. CRISPR/Cas9 plant expression vector for targeting phytoene desaturase (PsPDS) in pea The binary vector pKSE401 (Addgene, Plasmid #62,202), which contains Arabidopsis thaliana U6 promoter (AtU6) for gRNA expression and Zea mays codon-optimized Cas9 (ZmCas9) for efficient editing in pea, was used for designing a multiplex gRNAs construct targeting the first exon 1 3 12 Page 4 of 13 Plant Cell, Tissue and Organ Culture (PCTOC) (2025) 163:12 parameters were subsequently utilized for CRISPR/Cas9based editing of the phytoene desaturase (PsPDS) gene in pea protoplasts.



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